Generuler 100 bp plus dna ladder thermo fisher scientific. Pdf universal protocol for generating 100bp size standard for. The 100 bp dna ladder contains 11 discrete dna fragments ranging in size from 100 bp to 3,000 bp. The approximate mass of dna in each band is provided 0.
The 500 bp and 1,500 bp bands contain more dna to provide internal orientation. Invitrogen low mass dna ladder invitrogen low mass dna ladder. Other notes contains 10 bands, 1001,000 bp in exact 100 bp spaced ladder. By the use of these products you accept the terms and conditions of all applicable limited use label licenses. Sigmas pcr 100 bp low marker contains 10 dsdna fragments from 100 1,000 bases, evenly spaced in 100 bp repeats. The 500 bp band with higher concentration is distinguished.
The dna includes fragments ranging from 1001,500 base pairs. It consists of 15 bluntended fragments at 100 bp increments and is suitable for sizing doublestranded dna from 100 to 1,500. It contains bromophenol blue dye which serves as visual aid to monitor the progress of migration during agarose gel electrophoresis. Prepared from a plasmid containing repeats of a 25bp dna fragment, the ladder consists of 19 blunt fragments ranging in length from 25 to 450bp, at 25bp increments, plus a 500bp band. Thermo scientific generuler 100 bp plus dna ladder is designed for sizing and approximate quantification of wide range doublestranded dna on agarose and polyacrylamide gels. Identification of the preparationsubstance and of the company preparation name. The ladder is premixed with gel load buffer color dyes with varying mobility on an agarose gel for direct transfer to the gel. Ladder consists of 15 bluntended fragments ranging in length from 100 to 1,500 bp, at 100 bp increments, and an additional fragment at 2,072 bp.
Prepared from a plasmid containing repeats of a 100 bp dna fragment. For technical questions about this product, call the invitrogen techlinesm u. Use the 1 kb dna extension ladder to size linear doublestranded dna fragments from 500 bp40 kb. Band 600 bp two to three times brighter than other bands in ladder. Our pcrsizer contains ten discrete fragments ranging. Md121221 that are suitable as molecular weight standards for 100 bp dna ladder cat. The standard 100 base pairs bp dna ladder is an essential molecular marker in the molecular biology laboratory. Perfect 50% gc content eliminates spurious migration due to sequence.
Invitrogen 15628019 view more versions of this product. The tracking dyes do not obscure visualization of dna bands in ladder as the dyes run outside the limits of most dna bands in ladder. Sigmas directload pcr 100 bp low ladder is provided in a solution of 5% glycerol, 4. Consists of 15 fragments in 100bp increments ranging in size from 100 1,500bp and additional band at 2,072bp. Invitrogen 100 bp dna ladder 50ug invitrogen 100 bp dna. Our partners supplying these markers are norgen and biovision. The 100 bp dna ladder, containing 12 linear doublestranded dna fragments, is suitable for sizing doublestranded dna from 100 bp to 3,000 bp. Perfect 50% gc content eliminates spurious migration due to sequence variation, ensuring that the fragments migrate exactly according to their specified size.
Invitrogen trackit 100 bp dna ladder is ready to use and designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 2,000 bp. The norgen pcrsizer 100 bp dna ladder is prepared to ensure quality and batchtobatch consistency. Schematic representation of generating 100bp ladder. Consists of 15 fragments in 100bp increments ranging in. The invitrogen trackit 100 bp dna ladder is designed for sizing and quantification of double stranded dna on 1% to 2% agarose gels. Life technologies corporation andor it affiliates warrant their products as set forth in the life. Agarose gel analysis shows that the bands between 100 bp and 1,500 bp are accurate in size and distinguishable. This ladder was not designed for precise quantification of dna mass but can be used for approximating the mass of dna in comparably intense samples of similar size.
The 600 bp band is approximately 2 to 3 times brighter than the other ladder bands to provide internal orientation. Pdf universal protocol for generating 100bp size standard. Universal protocol for generating 100bp size standard for endless usage. It consists of 15 bluntended fragments at 100 bp increments and. Biolabs, stratagene, fermentas and invitrogen with varied characters.
The 100 bp dna ladder is suitable for sizing doublestranded dna from 100 to 1,500 bp. The 600 bp band is approximately 2 to 3 times brighter than the other ladder bands to. For optimal resolution, the recommended agarose gel concentration is 2. This marker is ideal for the size determination of pcr products. Use our molecular ruler to accurately assign molecular weights to dna fragments separated by gel electrophoresis. The readytouse 100 bp plus dna ladder comes premixed with loading buffer dye and ranges between 3000 to 100 bp. Two readytouse dna size markers for gel electrophoresis, the 100 bp ladder and 1 kb ladder represent the two most poplar markers used for dna fragment size determination. The 100 bp plus dna ladder consists of 19 double stranded molecular. Supplied with free vial of gel loading dye, purple 6x. Trackit 100 bp dna ladder thermo fisher scientific. The 100 bp plus dna ladder consists of 19 double stranded molecular weight markers, ranging in size from 100 to 1,000 bp in 100 bp increments, and additional fragments of 1,200, 1,600, 2,000, 2,961, 4,000, 5,007, 5,991, 8,000, 10,200 bp. High range dna ladder 500 bp 10 kb blue blue ngl 500 100 5x 500 500 m 204 m224 50 bp dna ladder 3. The intensity of 500 bp is about three times than the other bands to serve as reference points.
We currently have two different kinds of ladder in the lab. Pcr 100 bp low ladder for electrophoresis of pcr fragments. The dna ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 12 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The 500 bp band with higher concentration is distinguished easily from others. Ladder consists of 15 bluntended fragments ranging in length from 100 to 1,500 bp, at 100 bp increments, and an. Suitable for size determination of pcr generated dna. The 100 bp dna ladder is a unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and pcr products to. Prepared from a plasmid containing repeats of a 100 bp dna fragment, the ladder consists of.
Easy method for production of a homemade dna ladder in every. The following file naming structure is used to name the majority of these document files. C description the low dna mass ladder is composed of an equimolar mixture of six bluntended dna fragments of 2000, 1200, 800, 400, 200 and 100 bp. The ladder is generated from pcr and restriction enzyme digestion of double stranded dna. Generuler 100 bp plus dna ladder catalog number sm0321, sm0322 pub. Other notes contains 10 bands, 1001,000 bp in exact 100 bp spaced ladder recombinant repeats. Sigmas pcr 100 bp low marker is provided in a solution of 10 mm trishcl ph 8. Suitable for sizing doublestranded dna from 100 to 1,500bp. The bands of the ladder contain fragments following sizes. The invitrogen 100 bp dna ladder is designed for sizing and quantification of double stranded. Universal protocol for generating 100bp size standard for. Invitrogen 100 bp dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 2,000 bp. Unstained and fluorescent dna ladders jena bioscience. Quickload 100 bp dna ladder is a premixed, readytoload molecular weight marker containing bromophenol blue as a tracking dye.
Invitrogen trackit 100 bp dna ladder 100 applications. Invitrogen low mass dna ladder invitrogen low mass dna. Consists of 15 fragments in 100bp increments ranging in size from 1001,500bp and additional band at 2,072bp. The ladder is composed of fourteen chromatographypurified individual dna fragments in base pairs. Invitrogen 100 bp dna ladder 15628019 print share invitrogen 100 bp dna ladder. Invitrogen trackit 100 bp dna ladder fisher scientific. Prepared from a plasmid containing repeats of a 100 bp dna fragment, the ladder consists of 15 bluntended fragments ranging in length from 100 to 1,500 bp, at 100. Invitrogen 25 bp dna ladder 50ug nucleic acid molecular. This standard dna marker is normally used to determine the size of dna. Electrophoresis of 4 l of the low dna mass ladder results in bands containing 200, 120, 80, 40, 20, and 10 ng of dna, respectively. Contents amount storage sm0321 generuler 100 bp plus dna ladder 50 g for 100 applications, 0. Description the low dna mass ladder is composed of an equimolar mixture of six bluntended dna fragments of 2000, 1200, 800, 400, 200 and 100 bp. This ladder can be stained with ethidium bromide or radiolabeled using t4 dna polymerase or t4 polynucleotide kinase. Thermo scientific generuler 100 bp dna ladder is recommended for sizing and approximate quantification of a doublestranded dna in the range of 100 bp to.
Nucleic acid ladder is used as a size standard in agarose gel electrophoresis. The ladder is premixed with gel load buffer color dyes with. Readytoload 100 bp plus dna ladder ranging from 100 to. Thermo scientific generuler 100 bp dna ladder is designed for sizing and approximate quantification of wide. Electrophoresis of 4 l of the low dna mass ladder results in bands. Plus dna ladder if you need size markers for fragments between 100 bp and 12.
Can be visualized on 1% to 2% agarose gels after ethidium bromide staining. The readytouse 100bp ladder, premixed with loading buffer dye, ranges from 1,500 to 100 bp and has intense reference bands at 1500bp and 500 bp. Generuler 100 bp dna ladder thermo fisher scientific. Determination of the molecular weight mw or the base pair bp length. A proprietary plasmid is digested to completion with appropriate restriction enzymes to yield 11 bands suitable for use as molecular weight standards for both agarose and polyacrylamide gel. The dna ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for.
Trackit 100 bp dna ladder is premixed with sample buffer for readytoload convenience and room temperature storage. If samples need to be diluted, use te or other buffer of minimal ionic strength. The 500 and 1,500 base pair bands have increased intensity to serve as reference points. This ladder consists of bands containing 18 repeats of a 1018bp dna fragment. Click chemistry, dna ladder, homemade, pcr, primer. The trackit 1 kb dna ladder is suitable for sizing linear. The 100 bp dna ladder ranges from 100bp 1517 bp and is supplied with a free vial of gel. The 100 bp dna ladder consists of individual chromatographypurified dna fragments ranging in size from 100 bp to 2,000 bp. The 100 bp dna ladder consists of 15 bluntended fragments between 100 and 1500 bp in multiples of 100 bp and an additional fragment at 2072 bp. This standard dna marker is normally used to determine the size of dna fragments. Quickload 100 bp dna ladder from new england biolabs. Tridye 1 kb dna ladder is a premixed, readytoload molecular weight marker containing 3 dyes which serve as visual aids to monitor the progress of migration during agarose gel electrophoresis.
626 584 72 1396 118 305 532 551 767 222 478 175 668 348 1418 1389 127 252 495 1045 791 1275 1387 900 1277 70 1426 78 1300 1481 619 667 271 934